High-throughput linkage analysis ofMutatorinsertion sites in maize
نویسندگان
چکیده
منابع مشابه
High-throughput linkage analysis of Mutator insertion sites in maize.
Insertional mutagenesis is a cornerstone of functional genomics. High-copy transposable element systems such as Mutator (Mu) in maize (Zea mays) afford the advantage of high forward mutation rates but pose a challenge for identifying the particular element responsible for a given mutation. Several large mutant collections have been generated in Mu-active genetic stocks, but current methods limi...
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High-copy transposon mutagenesis is an effective tool for creating gene disruptions in maize. In order to molecularly define transposon-induced disruptions on a genome-wide scale, we optimized TAIL-PCR to amplify genomic DNA flanking maize Robertson's Mutator insertions. Sample sequencing from 43 Mutator stocks and the W22 inbred line identified 676 non-redundant insertions, and only a small fr...
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* Corresponding author. E-mail address: [email protected] (T.E. Gr 1537-5110/$ e see front matter a 2011 IAgrE doi:10.1016/j.biosystemseng.2011.06.004 This paper describes the development of high-throughput measurement techniques allowing acquisition of phenotypical data describing maize roots. One of a maize root’s traits is the level of complexity, which was expressed in a Fractal Dimension ...
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Linkage maps enable the study of important biological questions. The construction of high-density linkage maps appears more feasible since the advent of next-generation sequencing (NGS), which eases SNP discovery and high-throughput genotyping of large population. However, the marker number explosion and genotyping errors from NGS data challenge the computational efficiency and linkage map qual...
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BACKGROUND Aluminum (Al) toxicity is a major worldwide constraint to crop productivity on acidic soils. Al becomes soluble at low pH, inhibiting root growth and severely reducing yields. Maize is an important staple food and commodity crop in acidic soil regions, especially in South America and Africa where these soils are very common. Al exclusion and intracellular tolerance have been suggeste...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2009
ISSN: 0960-7412,1365-313X
DOI: 10.1111/j.1365-313x.2009.03821.x